Learning Outline

Blood

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A&P 2

Overview of blood tissue

Introduction

Blood connects all the various tissues of the body

  • Blood is part of every organ and every system
  • Is a “connective” tissue in a functional sense
  • Will relate directly to remaining topics of this course: cardiovascular, respiration, etc.

Structure

Connective tissue with liquid matrix (plasma)

Cells and cell fragments are also called formed elements

Flows within cardiovascular system (heart and blood vessels)

Blood volume

  • 71 ml/kg (70 kg adult male would have about 5,000 ml total blood volume)
  • About 55% is fluid and about 45% formed elements

Function

Primary functions slide

  • Transportation of molecules within the internal fluid environment
  • Exchange between tissues and between internal/external environment

Secondary functions

  • Immunity — defense
  • Thermoregulation — acts as “radiator fluid” tv icon
  • Fluid volume homeostasis
  • pH homeostasis

Blood and cardiovascular problems are a major health concern slide

lion trackFYI Interested in learning more? I recommend this book for further reading—Five Quarts: A Personal and Natural History of Blood

Formed elements – overview

Hematopoiesis

Hematopoiesis — formation of new blood cells

  • All are descended from stem cells in red marrow (myeloid tissue)
  • Divide into “lines” of daughter cells, each line producing different groups of blood cells (differentiation)

Main types

  • Platelets — clotting cells
  • Red blood cells — carry blood gases
  • White blood cells — immune cells

lion trackFYI Cells of the blood is a visualization of each type of blood cell.

blood cells SEM

Blood cell types
Red cell (left), platelet (center), white cell (T lymphocyte; right). SEM

Red blood cells

Name

Also called RBCs or erythrocytes [lit. “red cells”]

Number

4-6 million per cubic mm of blood (RBC count)

45% of total blood volume (packed cell volume [PCV] or hematocrit)

hematocrit Hematocrit
(PCV “packed cell volume”)

Shape

Biconcave disk activity image

  • 7 micron (µm) diameter

Deformable

  • PGE increases deformability
  • PGE2 decreases deformability

Crenation — shrinkage from water loss (as in a hypertonic solution) picture icon

Schizocyte — broken cell (schizo = “split”) picture icon

RBC inside capillary RBC inside blood capillary.
The RBC must deform to flow through this tiny blood vessel.

Cytoplasm

Organelles few in number (no nucleus)

  • Lost during development

Contain about 300 million molecules of hemoglobin (Hb)

  • Quaternary protein made of four polypeptides, each with an iron (Fe) containing heme group in the center (of each folded polypeptide)
  • Oxyhemoglobin (HbO2) forms when O2 combines with heme groups
  • Carbaminohemoglobin (HbCO2) forms when CO2 combines with amino acids in polypeptide chains
  • Anemia — less normal hemoglobin than usual (< 10g/100ml)
    • Normal male: 14-16 g/dl (g/dl = g/100ml)
    • Normal female: 12-14 g/dl
    • Examples
      • Sickle cell anemia image tv icon
      • Pernicious anemia
      • Fe-deficiency anemia (target cells/codocytes)

Life cycle

RBC hematopoiesis

  • Erythropoiesis
    • Stem cells (hemocytoblasts) in red bone marrow (myeloid tissue)
  • Stimulated by hormone erythropoietin (EPO), which is formed in kidney in response to low O2 delivery to tissue cells (tissue hypoxia)
  • Example: hematocrit (RBC%) goes UP when you stay at higher elevations, where O2 concentration in air is lower
  • Requires: vitamin B12 (and intrinsic factor), folic acid (folate or vitamin B9), iron (Fe)
  • Blood doping
    • Add blood to increase hematocrit, and thus O2 capacity
    • Add EPO to increase RBC production, increasing O2 capacity

RBC destruction

  • Last about 4 months (can’t repair themselves because they lack nuclei)
  • Run through “obstacle courses” in spleen and liver and aged cells break apart
  • Macrophages eat them
  • Globin (polypeptide) part is reduced to amino acids and recycled
  • Heme is stripped of its iron, which is recycled
    • Remain part of heme is converted to bilirubin (yellowish pigment that gives plasma its characteristic “straw” color)

Formation of blood cells
The RBCs in this diagram look strange. What is odd about them?

Blood types

Blood typing is the earliest form of “tissue typing”

  • Karl Landsteiner discovered ABO system (1902), Rh system (1904) and later other blood types, for which he won a Nobel Prize

Based on glycoprotein markers (antigens) on surface of RBCs

ABO system looks for A or B markers (antigen)

  • Type A — antigen A only
  • Type B — antigen B only
  • Type AB — both A and B antigens
  • Type 0 — neither A nor B antigen present

Rh (D antigen) system (named for Rhesus monkeys)

  • Rh positive — Rh (D) antigen present
  • Rh negative — Rh antigen absent

Other systems of blood typing exist —looking for different antigens (markers)

Read over textbook material on practical applications of this theory

White blood cells

Name

White blood cells are also called WBCs or leukocytes [lit. “white cells”]

Number

Less than 1% of whole blood

  • 5,000 – 10,000 per cubic mm of blood (WBC count)
  • Leukopenia — abnormally low WBC count
  • Leukocytosis — abnormally high WBC count
    • Often observed in leukemia (tumor of WBCs)

Life cycle

Leukopoiesis is development of new WBCs

  • Produced in bone marrow, but some migrate to lymphoid tissue for most of development (lymphoid tissue is found in lymph nodes, thymus gland, spleen, liver, elsewhere)

Lifespan is uncertain, perhaps 3 days to a year, depending on type

Location

Not confined to blood stream

Chemotaxis — move toward chemical attractants (chemotactic factors)released at site of injury/infection slide tv icon

Diapedesis — move between, or even through, other cells to get to their target

diapedesis of WBC Diapedesis of WBC across wall of blood vessel.

Functions

Immune functions of various sorts, for example

  • Phagocytosis
  • Release of cell toxins
  • Release of immune function regulators
  • More on immunity later (see Chapter 21)

Types

Types distinguished by how they stain with Wright’s stain —a mix of different cell stains activity

Differential WBC count — distinguished how many of each type of WBC in sample

Granulocytes (look granular when stained)

  • Basophils (stain with the basic stain in Wright’s mixture)
  • Eosinophils (stain with the acid stain called eosin)
  • Neutrophils (stain with a neutral stain)

Agranulocytes (do not look granular when stained)

  • Lymphocytes (small—8 microns (µm); develop in lymphoid tissue)
  • Monocytes (large—15-20 microns (µm); develop into macrophages)

WBC ratio
click image to enlarge

Platelets

Name

Also called thrombocytes

Structure

Tiny, irregular fragments activity

  • 2-3 microns (µm)

Do not have nuclei

Life Cycle

Thrombopoiesis is development of new platelets

  • Formed in red marrow
  • Fragments of larger ancestor cells
    • Megakaryoblasts develop into megakaryocytes, which each release 2-3 thousand tiny platelets

Life span — about a week

Regulated by hormone thrombopoietin

Function

Play important role in stopping leaks in vessels

More on this role follows

Stopping leaks

Overview

Hemostasis — process of stopping leakage of blood

Coagulation — part of hemostasis, it involves a complex process that eventually forms a blood clot to stop leaking blood

  • Also called the clotting mechanism
  • Involves a cascade of processes in which one step triggers another, then another picture icon
    • Opportunities at various steps to enhance or inhibit process

Process

Injury to blood vessel occurs, exposing collagen fibers in wall of blood vessel

Platelet plug forms (short-term fix)

  • Platelets have net positive charge on outside surface (recall membrane potentials)
  • Collagen has slightly negative charge, so platelets are attracted to site of injury
  • Platelets release thromboxane, making them sticky and also calling in more platelets (chemotaxis in action) picture icon

Blood clot (thrombus) forms (medium-term fix)

  • Thromboplastin is released from platelets (intrinsic mechanism) and damaged tissue cells (extrinsic mechanism)
    • This is an example of redundancy . . . often seen in safety mechanisms
  • Triggers conversion of prothrombin (plasma protein) into thrombin
  • Thrombin converts fibrinogen (plasma protein) into fibrin
  • Fibrin is stringy protein that traps cells and tightens to form a rather solid mass (clot)

Blood clot dissolves as new tissue replaces injured tissue (long-term fix)

  • Dissolution of clot starts when clot is formed (pre-programmed dissolution)
    • Otherwise, thrombus may break off and float away (an embolus) and block blood flow in brain, heart, lungs —-killing you
  • Plasmin is chemical largely responsible for dissolving fibrin

Clotting mechanism requires many different chemicals (clotting factors) tv icon

  • Clotting factors are often identified by Roman numerals (Factor I, Factor II, etc.)
  • If any one factor is missing (or abnormal), hemophilia (clotting disorder) is likely to result
Queen Victoria of Great Britain

Queen Victoria (1819-1901) of Great Britain had a defective gene for Clotting Factor VIII, which caused “royal hemophilia” in her son Leopold as well as many other of Victoria’s numerous royal descendants. Many of Victoria’s descendants married into other royal families, spreading the defective gene widely and greatly affecting world history. For example, Victoria’s great-grandson Alexis was the heir to the Russian throne and circumstances surrounding his successful treatment for pain by the controversial monk Rasputin may have triggered the timing of the downfall of the Czarist regime in Russia and thus events subsequent to the Russian revolution.

Today, people with abnormal Factor VIII can use powdered, freeze-dried CF VIII to help them cope with this disorder. Unfortunately, it was originally derived from human donor blood and risks blood-borne disease transmission including hepatitis and HIV. In fact, the HIV epidemic took a huge toll on hemophilia sufferers during the 1980s and 1990s. However, today synthetic (recombinant) sources of CF VIII and other therapies have made hemophilia treatments safer and more effective.

Plasma

Location

Extracellular liquid part of blood tissue

Composition

See diagram in text for specific proportions

Mostly water

Plasma protein

  • Albumins “thicken” blood and because they are relatively impermeant, hold water in the blood by way of osmosis
  • Globulins include immunoglobulins (Ig) —antibodies— for immunity
  • Fibrinogen is a protein needed for blood clotting

Other solutes

  • Ions such as Na+, Cl, K+, Ca++, H+, HCO3, and others
  • Nutrients such as glucose, fatty acids, amino acids
  • Waste products such as
    • Lactic acid from anaerobic processes
    • Urea from breakdown of amino acids used in cellular respiration
    • Yellowish pigments from breakdown of hemoglobin (from old red blood cells)
    • Gases such as CO2 and O2
  • Regulatory substances such as hormones, prostaglandins, clotting factors

Blood serum (pl. sera) — plasma without the clotting factors

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Last updated: November 29, 2016 at 5:23 am