Learning Outline

Mini Lesson: DNA

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Deoxyribonucleic acid—the information molecule

Structure of DNA

Nucleotides

Nucleotide is the subunit, made of three components:

  • Sugar (ribose in RNA; deoxyribose in DNA)
  • Phosphate
  • Nitrogen base
    • Different types of nitrogen base: cytosine, guanine, adenine, thymine (DNA only), uracil (RNA only)
    • First letters are used as abbreviations: C, G, A, T, U
    • Bases may link C-G or G-C and T-A or A-T (in RNA, U-A or A-U)
      • This rule is complementary or obligatory “base-pairing”

Nucleotides combine to form a double helix structure

  • Sugar and phosphate groups link together to form the “backbones” of the double helix
  • The nitrogen bases link in pairs to connect the two backbones

Linked nucleotides
Nucleotides link to form DNA.
Sugars and phosphates link to form the backbones and bases link in pairs to hold both helices together.
(Click image to enlarge it and view source)

dna structure
DNA double helix model.
(click image for larger view)

width=”150″ height=”148″ align=”left” hspace=”10″ />This classic photo of James Watson (l) and Francis Crick (r) shows them in 1953 with their original model for the double helix model of the DNA molecule.

Maurice Wilkins and Rosalind Franklin have also been given credit for participating in the discovery of this vitally important molecule’s structure and essential function. (Wilkins shared the 1962 Nobel Prize in Medicine/Physiology with Watson & Crick)

Researchers continue to unravel the many complexities and mysteries of what Watson called, “the most golden of all molecules.”

Genetish—language of genetics

Genetish is a made-up word introduced by author Matt Ridley to describe the coding used by cells in DNA/RNA

Codon

  • Every three bases make up one “word” or codon in genetish
  • Each different codon represents a different amino acid
    • Amino acids combine to form polypeptides or proteins

Gene

  • A sequence of codons containing the information needed to make one polypeptide/protein is called a gene (gene = “recipe” for one protein)
    • A gene could also make more than one type of polypeptide
    • A gene could instead make a functional RNA molecule
  • Genes are found at various locations along a DNA molecule (chromatin/chromosome)
  • All genes on all chromosomes is a set of information called the genome

Codon table. This table is a type of “phrasebook” for the language of “genetish” —-showing the meaning of different possible codons.

Codon wheel. This is another type of “decoder” for determining which amino acid is represented by any codon. (clickimage to enlarge it and view source)

liontrackClick here to see Ben Fry’s easier-to-read version of this classic table!
Ben constructed this as a student at MIT.
This version is similar to that found in our Anatomy & Physiology textbook (except that the textbook version is adapted to HUMAN genetish).

Function of DNA

The information molecule

Master code for proteins made by the cell activity

  • Proteins perform functions that regulate the cell, make up parts of the cell, and regulate the synthesis (and breakdown) of other types of molecules (lipids, carbohydrates, so on)

In humans, temporary “working copies” of specific genes from DNA are in the form of RNA (review Cell Structure & Function)

Information “between the genes” formerly called “junk DNA” may also have functions in regulating gene expression, etc.

Acronym Table
rRNA
ribosomal RNA
Forms ribosomes
mRNA
messenger RNA
Unfolded strand contains gene (code for one polypeptide); temporarily folds when leaving nucleus
tRNA
transfer RNA
Brings specific amino acids to ribosome and places them according to code on mRNA
nuclear DNA “Master” genetic code in the nucleus
mDNA or mtDNA mitochondrial DNA Additional “master” genetic code in the mitochondrion

lion track iconOptional: Click here for another helpful outline Making Proteins from the Pre-A&P course.

Replication of DNA

DNA helix “unzips” and nucleotides with bases complementary to those in each exposed DNA strand “fill in” and make a new side

  • Results in two identical “daughter molecules” of DNA
  • Semiconservative (half is new; half is old)

Facilitated by enzymes (isn’t everything?)

DNA replication
DNA replication.
(click image for source and attribution)

Transcription of RNA

Transcription video

mRNA is a “transcribed” copy of one gene in DNA

DNA unzips at one gene, and other side “fills in” with RNA nucleotides with bases that complement the exposed bases of the DNA strand

  • Promoter — sequence of bases that tells the cell where to start transcribing the gene
  • RNA polymerase — enzyme that facilitates formation of mRNA strand

transcription
Transcription
(click image to enlarge)

Editing

mRNA transcript is edited before leaving nucleus

  • Introns — parts of sequence that are deleted (“nonsense”)
  • Exons — parts of sequence the remain in the final mRNA molecule
  • A structure called the spliceosome forms on the mRNA, facilitating the splicing of transcript (removal of introns, gluing together of exons) video

DNA introns and exons
DNA editing (simplified)

(click image for larger view)

Other forms of RNA (tRNA, mRNA) produced in a similar manner

  • The edited transcript then usually folds into a complex shape, unlike the simple strand of mRNA

mRNA leaves nucleus via nuclear pores

Translation of RNA (protein synthesis) animation

Location

  • Translation occurs in the cytoplasm outside the nucleus

Process

  • Initiation — mRNA associates with rRNA of ribosome
  • Elongation — tRNA brings amino acids into place (anticodons on tRNA complement codons on mRNA) elongating string of amino acids
  • Termination — protein is released

translation
Translation.
Each tRNA (blue) brings a specific amino acid to ribosome (green) and links to the complementary codon on mRNA strand. As amino acids link together with peptide bonds, a polypeptide grows in length.

Mutations

Definition

  • Chromosomal mutations — additions, deletions to chromosome
  • Point mutations — change in one or few nucleotides in a gene sequence

Cause

  • Mutations can be spontaneous (no known cause)
  • Can be caused by radiation (x-ray, UV, etc.), chemicals, mechanical damage, extreme temperature, and other factors

Regulation of gene expression

Chromosome level

  • Packing — DNA wraps around histone proteins, forming “beads” called nucleosomes
  • Packing can prevent certain genes from being activated (transcribed)

Transcription level

  • Master genes turn on a group of other genes
  • Enhancer genes change the rate of transcription of other genes
  • Regulatory chemicals, such as steroid hormones, may activate certain genes
  • mRNA can be edited in different ways (exons can be linked in different ways)
  • mRNA can be “made ahead of time” and then “masked” until needed later

Translation level

  • Translation can be halted by regulatory mechanisms in the cell
  • Interferon released by nearby virus-infected cells can trigger the activation of genes that produce translation-inhibiting proteins
    • This causes the cell to be more careful in checking mRNA and will halt translation of viral RNA that has infected the cell
  • RNA interference (RNAi)
    • Mechanism by which translation of mRNA can be disrupted (interference)
    • May help regulate gene expression
    • May help defend against mutations and viral RNA infection
    • Also called gene silencing

Location of DNA

Nucleus

  • 46 chromosomes (diploid number) within nucleus (except during cell division)

align=”center”>DNA structure and location
DNA location and structure.

Mitochondrion

  • Single, ringlike strand of DNA comprising one chromosome

mitochondrial dnaMitochondrial DNA (mtDNA). This ringlike strand of DNA, similar to that seen in bacteria, contains genes that code for enzymes needed in mitochondrial function. Click on image to see larger source image, which is labeled with gene locations.

compass roseGenomic cartography is the study of methods to map out the genome of humans an other species in a way that makes the information easy to interpret and therefore more useful to everyone. Click here for an optional discussion from genomic cartographer Ben Fry.

If you think about it, DNA is kind of like a cookbook, with genes being the individual recipes used to “cook up” the proteins of the body!

bright idea iconKevin’s Mom’s Hot Milk Cake Recipe

bright idea iconKevin’s Favorite Blueberry Oatmeal Cookie Recipe

Want to learn more about the DNA-cooking analogy?
See Survival Guide for Anatomy & Physiology: Tips, Techniques, and Shortcuts

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Last updated: November 28, 2016 at 13:57 pm